iGUIDE: an improved pipeline for analyzing CRISPR
2019-1-17 · The iGUIDE method involves the same steps as in GUIDE-seq, but a larger ODN is used (46 nt versus 34 nt). As a result, the PCR primer binding sites can be moved away from the junction between the ODN and flanking human-derived DNA, leaving a segment of the ODN in the final PCR product.
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